Home > Sandwich ELISA Kits > Human Chemokine C-X-C-Motif Receptor 3 (CXCR3) ELISA Kit

Product Name:

Human Chemokine C-X-C-Motif Receptor 3 (CXCR3) ELISA Kit

Cat.#:

JL12547

Regulatory Status:

RUO

Alternative:

CD183; CKR-L2; CMKAR3; GPR9; IP10r; IP10-R; Mig-R; MigR; G Protein-Coupled Receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.06ng/mL

Specificity:

This kit recognizes Human CXCR3 in samples. No significant cross-reactivity or interference between Human CXCR3 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

Email *

Message *

Please Sign In.

Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| CXCR3

Chemokine receptor CXCR3 is a Gαi protein-coupled receptor in the CXC chemokine receptor family. Other names for CXCR3 are G protein-coupled receptor 9 (GPR9) and CD183. There are three isoforms of CXCR3 in humans: CXCR3-A, CXCR3-B and chemokine receptor 3-alternative (CXCR3-alt).CXCR3-A binds to the CXC chemokines CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (I-TAC) whereas CXCR3-B can also bind to CXCL4 in addition to CXCL9, CXCL10, and CXCL11.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.41	1.84	1.21	0.85	0.52	0.33	0.21	0.08
Corrected OD	2.33	1.76	1.13	0.77	0.44	0.25	0.13	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human CXCR3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	93-105	102
Tissue Homogenates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human CXCR3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108